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dmso differentiated hl60 cells  (ATCC)


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    ATCC dmso differentiated hl60 cells
    HVTN 124 mAb ADCP activity. HVTN 124 ADCP activity using native isotypes. ADCP activity was measured against HIV-1 BG505 T332N SOSIP gp140, HIV-1 CNE55 SOSIP gp140, influenza H1N1 A/New Caledonia/30/1999 HA, and SARS-CoV-2 D614G Spike. Antigens were each biotinylated and conjugated to fluorescent neutravidin beads, then mixed with each mAb. Antibodies are listed along the X-axis, while the Y-axis lists the ADCP score as AUC. The control mAbs used include the following: VRC01, PGT128 = HIV-1; Fe53, CR9114 = influenza; CR3022 = SARS-CoV-2. Palivizumab was included as a negative control antibody. Antibodies are color-coded by HVTN124 mAbs (blue), positive control mAbs (green), and negative control mAbs (red). For IgG isotypes, THP-1 monocytes were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. For IgA isotypes, <t>DMSO-differentiated</t> <t>HL60</t> cells were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. Antigen engulfment is indicated as an ADCP score. All experiments were performed in triplicate. Additional plots for the native IgA and non-native isotypes can be found in .
    Dmso Differentiated Hl60 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 7224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmso differentiated hl60 cells/product/ATCC
    Average 99 stars, based on 7224 article reviews
    dmso differentiated hl60 cells - by Bioz Stars, 2026-02
    99/100 stars

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    1) Product Images from "Glycan-reactive antibodies isolated from human HIV-1 vaccine trial participants show broad pathogen cross-reactivity"

    Article Title: Glycan-reactive antibodies isolated from human HIV-1 vaccine trial participants show broad pathogen cross-reactivity

    Journal: Journal of Virology

    doi: 10.1128/jvi.01256-25

    HVTN 124 mAb ADCP activity. HVTN 124 ADCP activity using native isotypes. ADCP activity was measured against HIV-1 BG505 T332N SOSIP gp140, HIV-1 CNE55 SOSIP gp140, influenza H1N1 A/New Caledonia/30/1999 HA, and SARS-CoV-2 D614G Spike. Antigens were each biotinylated and conjugated to fluorescent neutravidin beads, then mixed with each mAb. Antibodies are listed along the X-axis, while the Y-axis lists the ADCP score as AUC. The control mAbs used include the following: VRC01, PGT128 = HIV-1; Fe53, CR9114 = influenza; CR3022 = SARS-CoV-2. Palivizumab was included as a negative control antibody. Antibodies are color-coded by HVTN124 mAbs (blue), positive control mAbs (green), and negative control mAbs (red). For IgG isotypes, THP-1 monocytes were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. For IgA isotypes, DMSO-differentiated HL60 cells were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. Antigen engulfment is indicated as an ADCP score. All experiments were performed in triplicate. Additional plots for the native IgA and non-native isotypes can be found in .
    Figure Legend Snippet: HVTN 124 mAb ADCP activity. HVTN 124 ADCP activity using native isotypes. ADCP activity was measured against HIV-1 BG505 T332N SOSIP gp140, HIV-1 CNE55 SOSIP gp140, influenza H1N1 A/New Caledonia/30/1999 HA, and SARS-CoV-2 D614G Spike. Antigens were each biotinylated and conjugated to fluorescent neutravidin beads, then mixed with each mAb. Antibodies are listed along the X-axis, while the Y-axis lists the ADCP score as AUC. The control mAbs used include the following: VRC01, PGT128 = HIV-1; Fe53, CR9114 = influenza; CR3022 = SARS-CoV-2. Palivizumab was included as a negative control antibody. Antibodies are color-coded by HVTN124 mAbs (blue), positive control mAbs (green), and negative control mAbs (red). For IgG isotypes, THP-1 monocytes were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. For IgA isotypes, DMSO-differentiated HL60 cells were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. Antigen engulfment is indicated as an ADCP score. All experiments were performed in triplicate. Additional plots for the native IgA and non-native isotypes can be found in .

    Techniques Used: Activity Assay, Control, Negative Control, Positive Control, Flow Cytometry



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    dmso differentiated hl60 cells  (ATCC)
    99
    ATCC dmso differentiated hl60 cells
    HVTN 124 mAb ADCP activity. HVTN 124 ADCP activity using native isotypes. ADCP activity was measured against HIV-1 BG505 T332N SOSIP gp140, HIV-1 CNE55 SOSIP gp140, influenza H1N1 A/New Caledonia/30/1999 HA, and SARS-CoV-2 D614G Spike. Antigens were each biotinylated and conjugated to fluorescent neutravidin beads, then mixed with each mAb. Antibodies are listed along the X-axis, while the Y-axis lists the ADCP score as AUC. The control mAbs used include the following: VRC01, PGT128 = HIV-1; Fe53, CR9114 = influenza; CR3022 = SARS-CoV-2. Palivizumab was included as a negative control antibody. Antibodies are color-coded by HVTN124 mAbs (blue), positive control mAbs (green), and negative control mAbs (red). For IgG isotypes, THP-1 monocytes were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. For IgA isotypes, <t>DMSO-differentiated</t> <t>HL60</t> cells were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. Antigen engulfment is indicated as an ADCP score. All experiments were performed in triplicate. Additional plots for the native IgA and non-native isotypes can be found in .
    Dmso Differentiated Hl60 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmso differentiated hl60 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    dmso differentiated hl60 cells - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier

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    HVTN 124 mAb ADCP activity. HVTN 124 ADCP activity using native isotypes. ADCP activity was measured against HIV-1 BG505 T332N SOSIP gp140, HIV-1 CNE55 SOSIP gp140, influenza H1N1 A/New Caledonia/30/1999 HA, and SARS-CoV-2 D614G Spike. Antigens were each biotinylated and conjugated to fluorescent neutravidin beads, then mixed with each mAb. Antibodies are listed along the X-axis, while the Y-axis lists the ADCP score as AUC. The control mAbs used include the following: VRC01, PGT128 = HIV-1; Fe53, CR9114 = influenza; CR3022 = SARS-CoV-2. Palivizumab was included as a negative control antibody. Antibodies are color-coded by HVTN124 mAbs (blue), positive control mAbs (green), and negative control mAbs (red). For IgG isotypes, THP-1 monocytes were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. For IgA isotypes, DMSO-differentiated HL60 cells were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. Antigen engulfment is indicated as an ADCP score. All experiments were performed in triplicate. Additional plots for the native IgA and non-native isotypes can be found in .

    Journal: Journal of Virology

    Article Title: Glycan-reactive antibodies isolated from human HIV-1 vaccine trial participants show broad pathogen cross-reactivity

    doi: 10.1128/jvi.01256-25

    Figure Lengend Snippet: HVTN 124 mAb ADCP activity. HVTN 124 ADCP activity using native isotypes. ADCP activity was measured against HIV-1 BG505 T332N SOSIP gp140, HIV-1 CNE55 SOSIP gp140, influenza H1N1 A/New Caledonia/30/1999 HA, and SARS-CoV-2 D614G Spike. Antigens were each biotinylated and conjugated to fluorescent neutravidin beads, then mixed with each mAb. Antibodies are listed along the X-axis, while the Y-axis lists the ADCP score as AUC. The control mAbs used include the following: VRC01, PGT128 = HIV-1; Fe53, CR9114 = influenza; CR3022 = SARS-CoV-2. Palivizumab was included as a negative control antibody. Antibodies are color-coded by HVTN124 mAbs (blue), positive control mAbs (green), and negative control mAbs (red). For IgG isotypes, THP-1 monocytes were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. For IgA isotypes, DMSO-differentiated HL60 cells were subsequently added, and ADCP was calculated based on the engulfment of the antigen-coated beads by the THP-1 cells as measured by flow cytometry. Antigen engulfment is indicated as an ADCP score. All experiments were performed in triplicate. Additional plots for the native IgA and non-native isotypes can be found in .

    Article Snippet: These complexes were then incubated with either THP-1 cells (human monocyte cell line, ATCC TIB-201) for IgG antibodies or DMSO-differentiated HL60 cells (neutrophil-like cell line expressing IgA Fc receptors, ATCC CCL-240) for IgA antibodies.

    Techniques: Activity Assay, Control, Negative Control, Positive Control, Flow Cytometry